Ricky, I have to admit I have difficulty understanding your posts sometimes

next time can u write plain clear sentence pls?

I know that's probably how u usually write in the internet, but it makes things easier for us if u write in clear concise sentences and not run around. Just my two cents
As for your question, if u use water conditioner, u don't have to let the water sit overnight. The only reason we sit the water overnight is if we don't have any water conditioners available. The chlorine will evaporate by itself if u let the water sit for about 24 hours or more.